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a Schematic illustration of droplets required for <t>DropLab-based</t> <t>ELISA.</t> b Schematic of microbead-based ELISA on DropLab. c Images of the <t>CRP</t> ELISA in a high sample concentration ci and a low concentration cii . d Standard curve of an ELISA for di human IgG against SARS-CoV-2 S protein, dii CRP and diii TnC
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a Schematic illustration of droplets required for <t>DropLab-based</t> <t>ELISA.</t> b Schematic of microbead-based ELISA on DropLab. c Images of the <t>CRP</t> ELISA in a high sample concentration ci and a low concentration cii . d Standard curve of an ELISA for di human IgG against SARS-CoV-2 S protein, dii CRP and diii TnC
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a Schematic illustration of droplets required for <t>DropLab-based</t> <t>ELISA.</t> b Schematic of microbead-based ELISA on DropLab. c Images of the <t>CRP</t> ELISA in a high sample concentration ci and a low concentration cii . d Standard curve of an ELISA for di human IgG against SARS-CoV-2 S protein, dii CRP and diii TnC
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a Schematic illustration of droplets required for DropLab-based ELISA. b Schematic of microbead-based ELISA on DropLab. c Images of the CRP ELISA in a high sample concentration ci and a low concentration cii . d Standard curve of an ELISA for di human IgG against SARS-CoV-2 S protein, dii CRP and diii TnC

Journal: Microsystems & Nanoengineering

Article Title: DropLab: an automated magnetic digital microfluidic platform for sample-to-answer point-of-care testing—development and application to quantitative immunodiagnostics

doi: 10.1038/s41378-022-00475-y

Figure Lengend Snippet: a Schematic illustration of droplets required for DropLab-based ELISA. b Schematic of microbead-based ELISA on DropLab. c Images of the CRP ELISA in a high sample concentration ci and a low concentration cii . d Standard curve of an ELISA for di human IgG against SARS-CoV-2 S protein, dii CRP and diii TnC

Article Snippet: The capture antibody for ELISA detection was prepared by labeling mouse anti-human CRP antibody (SEK11250, 11250-MM07T, Sino Biological) with biotin using the biotin conjugation kit, and the antibody concentration after labeling was 0.8 mg/mL.

Techniques: Enzyme-linked Immunosorbent Assay, Concentration Assay